ATP1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP1A1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

160

Rezensionen (2)

Kat-Nr. : 14418-1-AP

Synonyme

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

---

• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis using 14418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14418-1-AP

  37°C incubated HEK-293 membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14418-1-AP

  37°C incubated mouse brain total extract lysate and membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14418-1-AP

  WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 14418-1-AP

  IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:300) with HEK-293 cells lysate 2800ug.

• 

  IP experiment of A431 using 14418-1-AP

  IP Result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200ug.

• 

  IHC staining of human liver cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of Caco-2 using 14418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 14418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

"ATP1A1 Antibodies" Comparison

View side-by-side comparison of ATP1A1 antibodies from other vendors to find the one that best suits your research needs.

Compare now

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, bei 37°C inkubiertes Maushirngewebe, HEK-293-Zellen, Rattenherzgewebe
• Erfolgreiche IP: HEK-293-Zellen, A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Caco-2-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:400
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 142 publications below
• IHC: See 8 publications below
• IF: See 20 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14418-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATP1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1A1 fusion protein Ag5763
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC050359
• Gene symbol: ATP1A1
• Gene ID (NCBI): 476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Publikationen

Species	Application	Title
mouse	IF	Nature; Low-dose metformin targets the lysosomal AMPK pathway through PEN2.; Authors - Teng Ma
	WB	Adv Sci (Weinh); Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis; Authors - Wenqi Sun
human	WB	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li
human	WB	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
human	WB	Nat Commun; A broadly neutralizing humanized ACE2-targeting antibody against SARS-CoV-2 variants.; Authors - Yanyun Du
human	WB	Nat Commun; Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.; Authors - Yanyun Du

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Aleix (Verified Customer) (04-26-2022)

We tested it in immunofluorescence with skin frozen sections. It worked, although the signal is weak.

• Applications: Immunofluorescence
• Cell Tissue Type: Skin

FH

Gongsheng (Verified Customer) (12-18-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human epithelium

ATP1A1 Antibody Immunofluorescence, validation (1:100 dilution) in Human epithelium (Cat no:14418-1-AP)

Verwandte Produkte

ATP1A2 Polyclonal antibody

16836-1-AP

ATP1B1 Polyclonal antibody

15192-1-AP

ATP1B2 Polyclonal antibody

22338-1-AP