ARL8B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARL8B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 13049-1-AP

Synonyme

GIE1, EC:3.6.5.2, ARL10C, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 13049-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13049-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13049-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13049-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARL8B antibody (13049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Produktinformation

13049-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA ARL8B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL8B fusion protein Ag3711
• Vollständiger Name: ADP-ribosylation factor-like 8B
• Berechnetes Molekulargewicht: 186 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC013131
• Gene symbol: ARL8B
• Gene ID (NCBI): 55207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Lysosomal positioning coordinates cellular nutrient responses.; Authors - Korolchuk Viktor I VI ; KD Validated
human	WB	Nat Commun; DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy; Authors - Rahul Kumar
monkey	WB	Sci Adv; The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.; Authors - Meng Lu ; KD Validated
mouse	WB,IF	Sci Adv; Endosomal sorting drives the formation of axonal prion protein endoggresomes.; Authors - Romain Chassefeyre ; KO Validated
human	WB	Nat Commun; RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.; Authors - Tal Keren-Kaplan
mouse	WB	J Cell Biol; The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.; Authors - Natalia Schiefermeier

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olivia (Verified Customer) (01-08-2020)

WB: Lysates from mouse bone marrow-derived macrophages were subjected to SDS PAGE followed by western blot with 13049-1-AP(Arl8b antibody) at a dilution of 1:500 overnight at 4°C.IF: Mouse bone marrow-derived macrophages were fixed in 4% PFA and permeabilized with 0.5% saponin before incubation with 1:500 Arl8b antibody (in 1% BSA/0.1% saponin/PBS) overnight at 4°C, followed by incubation with anti-rabbit Alexa Fluor 568 for 1h at room temperature.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse bone marrow-derived macrophages

ARL8B Antibody Western Blot,Immunofluorescence, validation (1:500 dilution) in Mouse bone marrow-derived macrophages (Cat no:13049-1-AP)

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