androgen receptor Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

androgen receptor Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

42

Rezensionen (3)

Kat-Nr. : 22089-1-AP

Synonyme

AIS, androgen receptor, AR, DHTR, Dihydrotestosterone receptor, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 22089-1-AP

  WB result of androgen receptor antibody (22089-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-androgen receptor transfected MCF-7 cells.

• 

  WB analysis using 22089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22089-1-AP (androgen receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22089-1-AP (androgen receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 22089-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22089-1-AP (androgen receptor antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22089-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22089-1-AP (androgen receptor antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody (22089-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human prostate cancer using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody (22089-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human prostate cancer using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody (22089-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of LNCaP using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (22089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of LNCaP using 22089-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using androgen receptor antibody (22089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HepG2-Zellen, Mausherzgewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Prostatakarzinomgewebe, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 32 publications below
• IHC: See 10 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

22089-1-AP bindet in WB, IP, IHC, IF, ELISA androgen receptor und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: androgen receptor fusion protein Ag17291
• Vollständiger Name: androgen receptor
• Berechnetes Molekulargewicht: 914 aa, 99 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa, 110-120 kDa
• GenBank-Zugangsnummer: BC132975
• Gene symbol: AR
• Gene ID (NCBI): 367
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1. What is the molecular weight of AR? Are there any isoforms of AR?

The molecular weight of full-length androgen receptor (AR-B) is 110 kDa. An additional variant, AR-A, has an 87 kDa size and lacks the N-terminal 187 amino acids of AR-A (PMID: 8108393). Recently, more splice variants of AR have been discovered, raising protein products of around 80 kDa length (PMID: 19244107), as well as an AR45 variant of 45 kDa size (PMID: 15634333). AR splice variants differ in their cell line-specific expression (PMID: 24570075).

2. What is the subcellular localization of AR?

AR can be present in either or both of the cytoplasm and nucleus. In androgen-deprived cells, AR is found predominantly in the cytoplasm, while stimulation by androgens causes enrichment of androgen-bound AR in the nucleus. AR shuttles between the cytoplasm and nucleus and its phosphorylation state has an impact on the subcellular localization (PMID: 16282370).

3. Is AR post-translationally modified?

Post-translational modifications of the AR include phosphorylation, acetylation, methylation, SUMOylation, and ubiquitination (PMID: 21820033). These modifications have an impact on receptor stability, activity, and can change the observed molecular weight of the AR.

4. How to study AR signaling in cell culture?

It is important to control levels of cell stimulation while also looking at AR signaling. Fetal bovine serum (FBS) that is typically used in cell culture contains low levels of testosterone that are enough to stimulate the growth of prostate cells (PMID: 19676093), including the LNCaP cell line that is a commonly used human prostatic carcinoma cell model (PMID: 6831420). One possibility for complete testosterone deprivation is to use charcoal stripped FBS that removes lipophilic agents, including androgens. It is also not recommended to use phenol red in your medium because it is a weak estrogen (PMID: 3458212). Cell stimulation is often conducted by dihydrotestosterone (DHT).

5. What is the role of AR in prostate cancer?

AR plays a key role in the development and physiology of the prostate gland, and also cancer progression (PMID: 15082523). Mutations in AR altering ligands have been observed. The progression of the prostate cancer depends on AR activity and therefore blocking AR activity or lowering androgen levels is a key step related to androgen deprivation therapy (ADT).

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; SMAD3 promotes expression and activity of the androgen receptor in prostate cancer; Authors - Hee-Young Jeon
mouse	IHC	Oncogene; Identification of low-frequency variants of UGT1A3 associated with bladder cancer risk by next-generation sequencing.; Authors - Rui Zheng
human,mouse	WB,IHC	Biomed Pharmacother; Therapeutic targeting of histone lysine demethylase KDM4B blocks the growth of castration-resistant prostate cancer; Authors - Lingling Duan
mouse	IHC	Sci Total Environ; Imidacloprid disrupts the endocrine system by interacting with androgen receptor in male mice.; Authors - Xianling Yuan
mouse	WB,IF	Cancer Sci; Splicing factor PRPF6 upregulates oncogenic androgen receptor signaling pathway in hepatocellular carcinoma.; Authors - Huijuan Song
human	WB	Int J Biol Sci; PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.; Authors - Wei Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Antonio (Verified Customer) (03-27-2023)

This antibody worked better than expected. Better than several Same antibody from different company

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Emma (Verified Customer) (11-29-2021)

We couldn't get this antibody to work by IF on FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: Prostate

FH

Saswati (Verified Customer) (08-20-2019)

This antibody worked in a cell line with a lower expression of AR, when another antibody from another vendor did not work. I am very pleased with its earlier lot.Also, since this is a polyclonal antibody, many bands light up, but in my opinion these are legitimate bands.Also hoping that the quality control keeps good eye from one lot to another., I have yet to use the particular lot# I put in this note.Thanks

• Applications: Western Blot, Immunofluorescence, Flow Cytometry,
• Primary Antibody Dilution: 1:6000
• Cell Tissue Type: MDA-MB 231

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