Aquaporin 4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Aquaporin 4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

66

Kat-Nr. : 16473-1-AP

Synonyme

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, NMO-IgG, WCH4

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Galerie der Validierungsdaten

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  WB analysis using 16473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16473-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 16473-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16473-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16473-1-AP

  IP Result of anti-Aquaporin 4 (IP:16473-1-AP, 4ug; Detection:16473-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of rat brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of rat brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

• 

  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausherzgewebe, Maushirngewebe, Mausnierengewebe, Rattenherzgewebe, Rattenhirngewebe, SKOV-3-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Nierengewebe, Mausherzgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:2500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 16 publications below
• IF: See 26 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

16473-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Aquaporin 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Aquaporin 4 fusion protein Ag9561
• Vollständiger Name: aquaporin 4
• Berechnetes Molekulargewicht: 323 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa, 32-34 kDa
• GenBank-Zugangsnummer: BC022286
• Gene symbol: AQP4
• Gene ID (NCBI): 361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Publikationen

Species	Application	Title
human	IF	Brain; Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia.; Authors - Aiqing Chen
mouse	WB,IF	Brain Behav Immun; Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems.; Authors - Xinghua Liu
mouse	WB	Glia; Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.; Authors - Wenqiang Quan
mouse	WB	Brain Behav Immun; Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice.; Authors - Yanzhao Zhou
mouse	IF	J Neuroinflammation; Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder.; Authors - Xue Ma
rat	WB	J Neuroinflammation; Cottonseed oil alleviates ischemic stroke injury by inhibiting the inflammatory activation of microglia and astrocyte.; Authors - Min Liu

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