APOA4 Polyklonaler Antikörper

APOA4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 17996-1-AP

Synonyme

APOA4, apolipoprotein A IV

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 17996-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 17996-1-AP (APOA4 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17996-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17996-1-AP (APOA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17996-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17996-1-AP (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17996-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17996-1-AP (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

17996-1-AP bindet in WB, IHC, ELISA APOA4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOA4 fusion protein Ag12454
• Vollständiger Name: apolipoprotein A-IV
• Berechnetes Molekulargewicht: 396 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-46 kDa
• GenBank-Zugangsnummer: BC074764
• Gene symbol: APOA4
• Gene ID (NCBI): 337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

Publikationen

Species	Application	Title
human	IHC	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
		JCI Insight; The distinct metabolism between large and small HDL indicates unique origins of human apolipoprotein A4; Authors - Allison B Andraski
human	WB	Int J Mol Sci; Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112).; Authors - Sotaro Motoi
mouse	WB	J Proteomics; PMID: 31706944; Authors - Ni Yang
mouse	WB	Alcohol; Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.; Authors - Minjie Chen
human	WB	Cardiol Young; Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.; Authors - Li Zhang

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