AP repeat Polyklonaler Antikörper

AP repeat Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 24493-1-AP

Synonyme

AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP

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Galerie der Validierungsdaten

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  ELISA experiment of peptide using 24493-1-AP

  AP repeat Antibody (24493-1-AP) tested in ELISA. AP repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of AP repeat antibody (24493-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: peptide

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

24493-1-AP bindet in WB, IHC, IF, ELISA AP repeat und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: AP repeat
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.

Publikationen

Species	Application	Title
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku
human	WB, IHC	Acta Neuropathol; Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.; Authors - Ian R A Mackenzie
human	IF	Acta Neuropathol; Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.; Authors - Johnathan Cooper-Knock
human	WB,IF	Hum Mol Genet; Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.; Authors - Janis Bennion Callister
human	WB	J Cell Sci; SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.; Authors - Mirjana Malnar
human	WB	Life Sci Alliance; C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons.; Authors - Paolo M Marchi

Verwandte Produkte

C9orf72 Polyclonal antibody

22637-1-AP

C9orf72 Monoclonal antibody

66140-1-Ig

GR repeat Polyclonal antibody

23978-1-AP

GP repeat Polyclonal antibody

24494-1-AP

TDP-43 Polyclonal antibody

10782-2-AP