ANTXR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ANTXR2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16723-1-AP

Synonyme

anthrax toxin receptor 2, ANTXR2, CMG 2, CMG2, ISH, JHF

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 16723-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16723-1-AP (ANTXR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16723-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16723-1-AP (ANTXR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16723-1-AP

  IP Result of anti-ANTXR2 (IP:16723-1-AP, 4ug; Detection:16723-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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  IHC staining of human ovary using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16723-1-AP (ANTXR2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16723-1-AP (ANTXR2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16723-1-AP bindet in WB, IP, IHC, CoIP, ELISA ANTXR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANTXR2 fusion protein Ag10162
• Vollständiger Name: anthrax toxin receptor 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC107876
• Gene symbol: ANTXR2
• Gene ID (NCBI): 118429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANTXR2 (anthrax toxin receptor 2), also known as CMG2 (capillary morphogenesis gene 2 protein) is a transmembrane protein that is induced during capillary morphogenesis. It contains a vWA domain that shows strong binding to laminin and collagen IV, suggesting that this protein plays a role in basement membrane matrix assembly and endothelial cell morphogenesis (PMID: 11683410; 14508707). Like its paralog TEM8 (ANTXR1), ANTXR2 also functions as a receptor for anthrax toxin. Mutations in ANTXR2 result in the allelic disorders juvenile hyaline fibromatosis and infantile systemic hyalinosis (PMID: 14508707; 12973667; 22383261).

Publikationen

Species	Application	Title
human	WB	Angiogenesis; Capillary morphogenesis gene 2 (CMG2) mediates growth factor-induced angiogenesis by regulating endothelial cell chemotaxis.; Authors - Lorna M Cryan ; KO Validated
human	WB,IHC,CoIP	Oncogene; Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway.; Authors - Chengdong Ji
human	IHC	J Pathol; Capillary morphogenesis protein 2 is a novel prognostic biomarker and plays oncogenic roles in glioma.; Authors - Juan Tan
human	IHC	FASEB J; Genetic deletion of CMG2 exacerbates systemic-to-pulmonary shunt-induced pulmonary arterial hypertension.; Authors - Liukun Meng
Camelus bactrianus	WB	Reprod Domest Anim; Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.; Authors - Qi Wang
human	WB,IP	Proc Natl Acad Sci U S A; Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes.; Authors - Oksana A Sergeeva

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