GST tag Monoclonal antibody

GST tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum and More (1)

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3B5F8

Cat no : 66001-1-Ig

Synonyms



Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66001-1-Ig targets GST tag in WB, IP, CoIP, ELISA applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

Tested Reactivity recombinant protein, schistosoma japonicum
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberFN315687
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.

Publications

SpeciesApplicationTitle

Nat Microbiol

Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.

Authors - Hua Zhang
WB

Cell Res

Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.

Authors - Xingxing Xu
WB

Mol Cancer

SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis.

Authors - Haihua Yuan

Cell Res

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Authors - Shu-Xin Zhang
WB

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
WB

Nucleic Acids Res

SUMOylation of YTHDF2 promotes mRNA degradation and cancer progression by increasing its binding affinity with m6A-modified mRNAs.

Authors - Guofang Hou