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Alpha smooth muscle actin specific Recombinant antibody

Name: Alpha smooth muscle actin specific Recombinant antibody
Brand: Proteintech
SKU: 80008-1-RR

Alpha smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

CloneNo.

5H7

Review Product

Cat no : 80008-1-RR

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

Validation Data Gallery

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AllWBIHCIF-PIFFC (Intra)

WB analysis using 80008-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis using 80008-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 80008-1-RR
WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells.
WB analysis of zebrafish using 80008-1-RR
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis using 80008-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 80008-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse embryo using 80008-1-RR
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
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E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
FC experiment of C2C12 using 80008-1-RR
1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 80008-1-RR

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Tested Applications

Positive WB detected in	mouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue
Positive IHC detected in	human liver tissue, human heart tissue, human appendicitis tissue, mouse colon tissue, rat colon tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue, mouse embryo tissue
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 16 publications below
IHC	See 9 publications below
IF	See 9 publications below

Product Information

80008-1-RR targets Alpha smooth muscle actin specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

Tested Reactivity	human, mouse, rat, pig, chicken, zebrafish
Cited Reactivity	human, mouse, rat, pig, duck
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight	42 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	NM_001613
Gene Symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2882938
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protocols

Product Specific Protocols
WB protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol
IHC protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol
IF protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol
FC protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells Authors - Decai Wang View Article
mouse	WB,IHC	Pharmacol Res MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2. Authors - Yue Zhang View Article
human,mouse	WB,IHC,IF	Int Immunopharmacol TSLP/TSLPR promotes renal fibrosis by activating STAT3 in renal fibroblasts Authors - Decai Wang View Article
mouse	WB	Eur J Pharmacol Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway Authors - Shaoling Lin View Article
mouse	WB,IHC,IF	Drug Des Devel Ther Ginsenoside Rb1 Alleviates Bleomycin-Induced Pulmonary Inflammation and Fibrosis by Suppressing Central Nucleotide-Binding Oligomerization-, Leucine-Rich Repeat-, and Pyrin Domains-Containing Protein Three Inflammasome Activation and the NF-κB Pathway. Authors - Jingjing Liu View Article
mouse	IHC,IF	Exp Biol Med (Maywood) Targeting endothelial coagulation signaling ameliorates liver obstructive cholestasis and dysfunctional angiogenesis Authors - Xue Yang View Article

Alpha smooth muscle actin specific Recombinant antibody

Alpha smooth muscle actin specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 80008-1-RR

WB analysis using 80008-1-RR

WB analysis of A549 using 80008-1-RR

WB analysis of zebrafish using 80008-1-RR

WB analysis using 80008-1-RR

IHC staining of human liver using 80008-1-RR

IHC staining of human colon using 80008-1-RR

IHC staining of human colon using 80008-1-RR

IHC staining of human appendicitis using 80008-1-RR

IHC staining of human appendicitis using 80008-1-RR

IHC staining of human colon using 80008-1-RR

IHC staining of human heart using 80008-1-RR

IHC staining of human heart using 80008-1-RR

IHC staining of mouse colon using 80008-1-RR

IHC staining of rat colon using 80008-1-RR

IHC staining of human liver using 80008-1-RR

IF Staining of mouse heart using 80008-1-RR

IF Staining of mouse embryo using 80008-1-RR

FC experiment of C2C12 using 80008-1-RR

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2.

TSLP/TSLPR promotes renal fibrosis by activating STAT3 in renal fibroblasts

Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway

Ginsenoside Rb1 Alleviates Bleomycin-Induced Pulmonary Inflammation and Fibrosis by Suppressing Central Nucleotide-Binding Oligomerization-, Leucine-Rich Repeat-, and Pyrin Domains-Containing Protein Three Inflammasome Activation and the NF-κB Pathway.

Targeting endothelial coagulation signaling ameliorates liver obstructive cholestasis and dysfunctional angiogenesis

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Flow Cytometry Perm Buffer (10X)

Alpha smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA