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  • Featured Product
  • KD/KO Validated

WBP2 Monoclonal antibody

WBP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4G7

Cat no : 66585-1-Ig

Synonyms

WBP 2, WBP2, WW domain binding protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using WBP2 Monoclonal antibody (66585-1-Ig)

    WB analysis of HEK-293 using 66585-1-Ig

    WB result of WBP2 antibody (66585-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBP2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using WBP2 Monoclonal antibody (66585-1-Ig)

    WB analysis of HeLa using 66585-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using WBP2 Monoclonal antibody (66585-1-Ig)

    WB analysis of NIH/3T3 using 66585-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WBP2 Monoclonal antibody (66585-1-Ig)

    IHC staining of mouse testis using 66585-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WBP2 Monoclonal antibody (66585-1-Ig)

    IHC staining of mouse testis using 66585-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WBP2 Monoclonal antibody (66585-1-Ig)

    IF Staining of HeLa using 66585-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WBP2 Monoclonal antibody (66585-1-Ig)

    IF Staining of HeLa using 66585-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66585-1-Ig targets WBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WBP2 fusion protein Ag2661
Full Name WW domain binding protein 2
Calculated Molecular Weight 261 aa, 28 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC010616
Gene Symbol WBP2
Gene ID (NCBI) 23558
RRIDAB_2881945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WBP2 antibody 66585-1-IgDownload protocol
IHC protocol for WBP2 antibody 66585-1-IgDownload protocol
IF protocol for WBP2 antibody 66585-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Dis

WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.

Authors - Zhe Zheng
  • KO Validated
View Article