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KD/KO Validated

USP7 Polyclonal antibody

Name: USP7 Polyclonal antibody
Brand: Proteintech
SKU: 26948-1-AP

USP7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26948-1-AP

Synonyms

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

Validation Data Gallery

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AllWBIHC

WB analysis using 26948-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 26948-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 26948-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 26948-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 26948-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 26948-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 26948-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26948-1-AP (USP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 26948-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26948-1-AP (USP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 26948-1-AP

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Tested Applications

Positive WB detected in	MCF-7 cells, U2OS cells, K-562 cells, mouse spleen tissue, NIH/3T3 cells, PC-12 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 3 publications below
RIP	See 1 publications below

Product Information

26948-1-AP targets USP7 in WB, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP7 fusion protein Ag25634
Full Name	ubiquitin specific peptidase 7 (herpes virus-associated)
Observed Molecular Weight	126-128 kDa
Gene Symbol	USP7
Gene ID (NCBI)	7874
RRID	AB_2880696
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for USP7 antibody 26948-1-AP	Download protocol
IHC protocol for USP7 antibody 26948-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Cancer Res Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs. Authors - Monika Vishnoi View Article
human	WB	Front Immunol Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses. Authors - Wenying Gao KD Validated View Article
human	WB,IHC	Int J Biol Sci Flap endonuclease 1 Facilitated Hepatocellular Carcinoma Progression by Enhancing USP7/MDM2-mediated P53 Inactivation. Authors - Saiyan Bian View Article
human	WB	Discov Oncol High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer Authors - Haiqing Wang View Article
mouse	WB,IHC	Front Pharmacol Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice Authors - Yu-Hui Gu View Article
human	WB	ACS Pharmacol Transl Sci Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf Authors - Hongyue Li View Article

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