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UBP1 Monoclonal antibody

UBP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B10H3

Cat no : 67318-1-Ig

Synonyms

DKFZp686L1745, LBP 1a, LBP 1B, LBP1, LBP1A, LBP1B, Transcription factor LBP 1, UBP1, Upstream binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBP1 Monoclonal antibody (67318-1-Ig)

    WB analysis using 67318-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67318-1-Ig (UBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, K-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67318-1-Ig targets UBP1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBP1 fusion protein Ag5010
Full Name upstream binding protein 1 (LBP-1a)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC047235
Gene Symbol UBP1
Gene ID (NCBI) 7342
RRIDAB_2882578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBP1, also known as Transcription factor LBP-1, is a 540 amino acid protein, which is expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines. UBP1 localizes in nuclear and can be induced by HIV-1 infection of lymphocytes. UBP1 functions as a transcriptional activator in a promoter context-dependent manner and modulates the placental expression of CYP11A1. UBP1 is involved in regulation of the alpha-globin gene in erythroid cells and represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.

Protocols

Product Specific Protocols
WB protocol for UBP1 antibody 67318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols