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TLR2 Monoclonal antibody
TLR2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C1D3
Cat no : 66645-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells, U-937 cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
66645-1-Ig targets TLR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | TLR2 fusion protein Ag25626 |
Full Name | toll-like receptor 2 |
Calculated Molecular Weight | 784 aa, 90 kDa |
Observed Molecular Weight | 90-100 kDa |
GenBank Accession Number | BC033756 |
Gene Symbol | TLR2 |
Gene ID (NCBI) | 7097 |
RRID | AB_2882003 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TLR2 antibody 66645-1-Ig | Download protocol |
IHC protocol for TLR2 antibody 66645-1-Ig | Download protocol |
IF protocol for TLR2 antibody 66645-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Clin Transl Med Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease. | ||
Life Sci The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells | ||
Microbes Infect Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2 | ||
Front Microbiol PPARγ Ameliorates Mycobacterium tuberculosis H37Ra-Induced Foamy Macrophage Formation via the ABCG1-Dependent Cholesterol Efflux Pathway in THP-1 Macrophages. | ||
ACS Omega Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway. | ||
Virology p53 suppresses the inflammatory response following respiratory syncytial virus infection by inhibiting TLR2 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma.
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