• Featured Product
  • KD/KO Validated

TGFBR2 Monoclonal antibody

TGFBR2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2D5H7

Cat no : 66636-1-Ig

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2



Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, U2OS cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells, NCCIT cells, L02 cells, K-562 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66636-1-Ig targets TGFBR2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TGFBR2 fusion protein Ag25773
Full Name transforming growth factor, beta receptor II (70/80kDa)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-80 kDa
GenBank Accession NumberBC040499
Gene Symbol TGFBR2
Gene ID (NCBI) 7048
RRIDAB_2881995
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Protocols

Product Specific Protocols
WB protocol for TGFBR2 antibody 66636-1-IgDownload protocol
IHC protocol for TGFBR2 antibody 66636-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep Med

Engineering irradiated tumor-derived microparticles as personalized vaccines to enhance anti-tumor immunity

Authors - Yajie Sun
mouse,ratIF,WB,IHC

Acta Pharmacol Sin

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Authors - Ji-Chao Wu
ratWB,IP

Br J Pharmacol

Inhibition of HSP90 S-nitrosylation Alleviates Cardiac Fibrosis via TGFβ/SMAD3 Signaling Pathway.

Authors - Xiyue Zhang
ratIHC

NPJ Regen Med

Alkaline shear-thinning micro-nanocomposite hydrogels initiate endogenous TGFβ signaling for in situ bone regeneration

Authors - Yuting Niu
human,mouseWB,IHC

Acta Pharmacol Sin

N-n-Butyl haloperidol iodide ameliorates liver fibrosis and hepatic stellate cell activation in mice.

Authors - Dai-Fei Shen
  • KD Validated
humanWB,IP

Aging (Albany NY)

A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer.

Authors - Xiaotian Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kenzo (Verified Customer) (08-15-2023)

This antibody worked well for labeling TGF beta receptor type 2 in mouse kidney. We were able to acquire robust immunofluorescence signals with this KO validated antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Kidney tissue