mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67394-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67394-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 67394-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67394-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 67394-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 67394-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
FC experiment of HeLa using 67394-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:15000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67394-1-Ig targets RGS14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 67394-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 67394-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 67394-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67394-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 67394-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 67394-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 67394-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of HeLa using 67394-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RGS14 Monoclonal antibody
Catalog Number
67394-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:15000 IHC : 1:1000-1:4000 IF/ICC : 1:1000-1:4000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
