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KD/KO Validated

POLD3 Polyclonal antibody

Name: POLD3 Polyclonal antibody
Brand: Proteintech
SKU: 21935-1-AP

POLD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21935-1-AP

Synonyms

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3

Validation Data Gallery

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AllWBIPIHC

WB analysis using 21935-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21935-1-AP (POLD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 21935-1-AP
IP result of anti-POLD3 (IP:21935-1-AP, 4ug; Detection:21935-1-AP 1:500) with MCF-7 cells lysate 640 ug.
IHC staining of human breast cancer using 21935-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 21935-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 21935-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21935-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 21935-1-AP

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Tested Applications

Positive WB detected in	MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below

Product Information

The immunogen of 21935-1-AP is POLD3 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POLD3 fusion protein Ag16570
Full Name	polymerase (DNA-directed), delta 3, accessory subunit
Calculated Molecular Weight	466 aa, 51 kDa
Observed Molecular Weight	51-70 kDa
GenBank Accession Number	BC108908
Gene Symbol	POLD3
Gene ID (NCBI)	10714
RRID	AB_2878949
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLD3 antibody 21935-1-AP	Download protocol
IHC protocol for POLD3 antibody 21935-1-AP	Download protocol
IP protocol for POLD3 antibody 21935-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Genes Dev Break-induced replication promotes fragile telomere formation. Authors - Zhe Yang KO Validated View Article
mouse	WB	Genes Dev TRF1 uses a noncanonical function of TFIIH to promote telomere replication Authors - Zhe Yang KD Validated View Article
human	WB	J Adv Res Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA Authors - Xi Dong View Article
human		Mol Cell Mechanisms of insertions at a DNA double-strand break Authors - Jaewon Min View Article
mouse	WB	Nucleic Acids Res Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model Authors - Nada Kojak View Article
mouse	WB	Nucleic Acids Res Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres Authors - Jennifer J Lee View Article

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Research Area

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POLD3 Polyclonal antibody

POLD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 21935-1-AP

IP experiment of MCF-7 using 21935-1-AP

IHC staining of human breast cancer using 21935-1-AP

IHC staining of human cervical cancer using 21935-1-AP

IHC staining of human cervical cancer using 21935-1-AP

IHC staining of human breast cancer using 21935-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Break-induced replication promotes fragile telomere formation.

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA

Mechanisms of insertions at a DNA double-strand break

Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model

Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres