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  • Featured Product
  • KD/KO Validated

PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10495-1-AP

Synonyms

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using PIN1 Polyclonal antibody (10495-1-AP)

    WB analysis of HEK-293 using 10495-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIN1 Polyclonal antibody (10495-1-AP)

    WB analysis of HeLa using 10495-1-AP

    WB result of PIN1 antibody (10495-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PIN1 Polyclonal antibody (10495-1-AP)

    WB analysis using 10495-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PIN1 Polyclonal antibody (10495-1-AP)

    WB analysis of HEK-293 using 10495-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIN1 Polyclonal antibody (10495-1-AP)

    WB analysis of HeLa using 10495-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIN1 Polyclonal antibody (10495-1-AP)

    WB analysis of HeLa using 10495-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PIN1 Polyclonal antibody (10495-1-AP)

    IP experiment of HepG2 using 10495-1-AP

    IP result of anti-PIN1 (IP:10495-1-AP, 4ug; Detection:10495-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PIN1 Polyclonal antibody (10495-1-AP)

    IHC staining of human renal cell carcinoma using 10495-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PIN1 Polyclonal antibody (10495-1-AP)

    IHC staining of human renal cell carcinoma using 10495-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PIN1 Polyclonal antibody (10495-1-AP)

    IHC staining of human pancreas cancer using 10495-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PIN1 Polyclonal antibody (10495-1-AP)

    IHC staining of human pancreas cancer using 10495-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PIN1 Polyclonal antibody (10495-1-AP)

    IF Staining of HEK-293 using 10495-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10495-1-AP (PIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PIN1 Polyclonal antibody (10495-1-AP)

    IF Staining of HEK-293 using 10495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PIN1 Polyclonal antibody (10495-1-AP)

    IF Staining of HEK-293 using 10495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PIN1 Polyclonal antibody (10495-1-AP)

    IF Staining of NIH/3T3 using 10495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10495-1-AP targets PIN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIN1 fusion protein Ag0767
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC002899
Gene Symbol PIN1
Gene ID (NCBI) 5300
RRIDAB_2163943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

Protocols

Product Specific Protocols
WB protocol for PIN1 antibody 10495-1-APDownload protocol
IHC protocol for PIN1 antibody 10495-1-APDownload protocol
IF protocol for PIN1 antibody 10495-1-APDownload protocol
IP protocol for PIN1 antibody 10495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Nat Commun

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells

Authors - Qiuhong Zhu
View Article
human,mouseWB

J Pineal Res

Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.

Authors - Dongmei Chen
View Article
humanWB,IF

J Hazard Mater

Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.

Authors - Fuli Zheng
View Article
humanWB

Theranostics

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Authors - Ling Lin
View Article
humanWB

Nucleic Acids Res

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

Authors - Poolman Toryn M TM
  • KD Validated
View Article
mouseWB, IHC, IF

Oxid Med Cell Longev

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.

Authors - Yanzhuo Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other