CoraLite® Plus 488-conjugated PAX8 Monoclonal antibody
PAX8 Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4H7B3
Cat no : CL488-60145
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human ovary tumor tissue, mouse kidney tissue |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-60145 targets PAX8 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PAX8 fusion protein Ag0306 |
| Full Name | paired box 8 |
| Calculated Molecular Weight | 48 kDa |
| GenBank Accession Number | BC001060 |
| Gene Symbol | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_2883106 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PAX8 antibody CL488-60145 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
