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P16-INK4A Polyclonal antibody

P16-INK4A Polyclonal Antibody for WB, IP, FC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)


WB, IP, FC, ELISA, Cell treatment



Cat no : 10883-1-AP


ARF, ARF,CDKN2A, CDK4 inhibitor p16 INK4, CDK4 inhibitor p16INK4, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, P16-INK4A, TP16

Tested Applications

Positive WB detected inHEK-293 cells, HEK293 cells, HeLa cells, HepG2 cells, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10883-1-AP targets P16-INK4A in WB, IP, FC, ELISA, Cell treatment applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P16-INK4A fusion protein Ag1328
Full Name cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC021998
Gene Symbol CDKN2A
Gene ID (NCBI) 1029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information


p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.

 What is the molecular weight of P16?

16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.

What is the function of p16?

P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).

 What is the role of p16 in senescence?

In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.

 What is the role of p16 in cancer?

As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).


Product Specific Protocols
WB protocol for P16-INK4A antibody 10883-1-APDownload protocol
IHC protocol for P16-INK4A antibody 10883-1-APDownload protocol
IP protocol for P16-INK4A antibody 10883-1-APDownload protocol
FC protocol for P16-INK4A antibody 10883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Senolytic CAR T cells reverse senescence-associated pathologies.

Authors - Corina Amor


PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Authors - Federico Gulluni

Nat Genet

Early TP53 alterations engage environmental exposures to promote gastric premalignancy in an integrative mouse model.

Authors - Nilay S Sethi

Cancer Cell

mTORC1 Activation Blocks Braf(V600E)-Induced Growth Arrest but Is Insufficient for Melanoma Formation.

Authors - William Damsky


Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability.

Authors - Julie Wang

Cell Metab

SREBP1c-PARP1 axis tunes anti-senescence activity of adipocytes and ameliorates metabolic imbalance in obesity.

Authors - Gung Lee


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Vikas (Verified Customer) (04-23-2024)

Highly recommended, to use 1:1000 dilution for Western bloating.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y cell line

Vikas (Verified Customer) (04-23-2024)

Used p16 antibody for western-bolting at the dilution 1:10000, antibody worked at best. Thank you, highly recommended it.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y cell line

Susanne (Verified Customer) (10-11-2022)

blocking: ROTIblock Incubation over night 4°C

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 : 1000
  • Cell Tissue Type: mouse skin tissue

Malak (Verified Customer) (04-22-2021)

Unfortunately, with this antibody, I didn't get a bad at 16 KD, however it was higher (around 20 KD). The suppliers were so professional and offered their help to optimize this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Huh7 cells extract

Joshua (Verified Customer) (03-12-2020)

MDCKs fixed in 4% paraformaldehyde and stained overnight at 4 C. Excellent, bright stain.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MDCK
P16-INK4A Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:10883-1-AP)

Kyle (Verified Customer) (02-28-2019)

Clean bands, low background

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: small cell lung cancer