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Tested Applications
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
66781-1-Ig targets Neuromedin B in IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Neuromedin B fusion protein Ag0842 Predict reactive species |
| Full Name | neuromedin B |
| Calculated Molecular Weight | 16 kDa |
| GenBank Accession Number | BC007407 |
| Gene Symbol | NMB |
| Gene ID (NCBI) | 4828 |
| RRID | AB_2882126 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08949 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in pituitary gland, hypothalamus, pancreas and stomach. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo. (PMID: 15528253, PMID: 2458345).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Neuromedin B antibody 66781-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ann N Y Acad Sci The von Economo neurons in the frontoinsular and anterior cingulate cortex. |
