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  • KD/KO Validated

NPTX1 Polyclonal antibody

NPTX1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20656-1-AP

Synonyms

NP-I, NP1, Neuronal pentraxin-1, Neuronal pentraxin I



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, mouse liver tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20656-1-AP targets NPTX1 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name neuronal pentraxin I
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_002522
Gene Symbol NPTX1
Gene ID (NCBI) 4884
RRIDAB_10793669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPTX1, also named as NP1, may mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. NPTX1 can mediate the neuronal and glial uptake of the snake venom toxin taipoxin. The antibody is specific to NPTX1.

Protocols

Product Specific Protocols
WB protocol for NPTX1 antibody 20656-1-APDownload protocol
IHC protocol for NPTX1 antibody 20656-1-APDownload protocol
IF protocol for NPTX1 antibody 20656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, CoIP

Front Immunol

Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.

Authors - Réka Á Kovács
WB

Acta Biochim Biophys Sin (Shanghai)

miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells.

Authors - Kai Zhang
mouseIF

PLoS One

RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis.

Authors - Brianna Dufek
humanWB

Cancer Manag Res

NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.

Authors - Hongfei Yan
  • KD Validated
humanWB

PLoS One

Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis

Authors - Haijun Zhu
  • KD Validated
mouseWB

Oncogene

Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263

Authors - Lingyan Wang