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- KD/KO Validated
NFIL3 Polyclonal antibody
NFIL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 11773-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, K562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse stomach tissue, human skin cancer tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | NK92, NK92 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 14 publications below |
| IHC | See 5 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| ChIP | See 5 publications below |
Product Information
11773-1-AP targets NFIL3 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NFIL3 fusion protein Ag2380 |
| Full Name | nuclear factor, interleukin 3 regulated |
| Calculated Molecular Weight | 462 aa, 51 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC008197 |
| Gene Symbol | NFIL3 |
| Gene ID (NCBI) | 4783 |
| RRID | AB_2267287 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). The calculated molecular weight of NFIL3 is 51 kDa, but the modified NFIL3 is about 55-60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NFIL3 antibody 11773-1-AP | Download protocol |
| IHC protocol for NFIL3 antibody 11773-1-AP | Download protocol |
| IF protocol for NFIL3 antibody 11773-1-AP | Download protocol |
| IP protocol for NFIL3 antibody 11773-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice. | ||
Theranostics The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition. | ||
Neuropharmacology Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area. | ||
Biochem Pharmacol Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice | ||
Front Med (Lausanne) NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation. | ||
Drug Metab Dispos The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.
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