Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10723-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10723-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10723-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10723-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10723-1-AP
IP result of anti-KU70,XRCC6 (IP:10723-1-AP, 3ug; Detection:10723-1-AP 1:1000) with HeLa cells lysate 1600ug.
IHC staining of human colon cancer using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse kidney using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10723-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10723-1-AP
Immunofluorescent analysis of HepG2 cells, using KU70,XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 10723-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (10723-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HepG2 using 10723-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KU70,XRCC6 Polyclonal antibody (10723-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

KU70,XRCC6 Polyclonal antibody

Name: KU70,XRCC6 Polyclonal antibody
Brand: Proteintech
SKU: 10723-1-AP
Rating: 5.00 (1 reviews)

KU70,XRCC6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10723-1-AP

Publications(39)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

KU70, XRCC6, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, ATP-dependent DNA helicase 2 subunit 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (39)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human renal cell carcinoma tissue, human colon cancer tissue, human lung cancer tissue, mouse kidney tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 35 publications below
IHC	See 3 publications below
IF	See 9 publications below
IP	See 4 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

Product Information

10723-1-AP targets KU70,XRCC6 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1043 Product name: Recombinant human KU70,XRCC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 307-609 aa of BC008343 Sequence: TGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD Predict reactive species
Full Name	X-ray repair complementing defective repair in Chinese hamster cells 6
Calculated Molecular Weight	75 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC008343
Gene Symbol	KU70
Gene ID (NCBI)	2547
RRID	AB_2218756
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12956
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Protocols

Product Specific Protocols
IF protocol for KU70,XRCC6 antibody 10723-1-AP	Download protocol
IHC protocol for KU70,XRCC6 antibody 10723-1-AP	Download protocol
IP protocol for KU70,XRCC6 antibody 10723-1-AP	Download protocol
WB protocol for KU70,XRCC6 antibody 10723-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis Authors - Kaiwen Bao View Article
human	WB,IF	Oncogene UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy. Authors - L Hu View Article
human	WB	Phytomedicine Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer Authors - Meiyang Chen View Article
human	WB	J Mater Chem B Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer Authors - Shuying Li View Article
human	IF	Mol Med Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein Authors - Luping Zhang View Article
human	WB	FEBS J Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy Authors - Yan Wei View Article