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Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10590-1-AP

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of A431 using 10590-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of rat skin using 10590-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of MCF-7 using 10590-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of A375 using 10590-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of U-251 using 10590-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of MCF-7 using 10590-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    WB analysis of HeLa using 10590-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human oesophagus cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human oesophagus cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human lung cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human lung cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human breast cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human breast cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human skin using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IHC staining of human skin using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IF Staining of A431 using 10590-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 6A antibody (10590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IF Staining of A431 using 10590-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10590-1-AP (Cytokeratin 6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    IF Staining of HeLa using 10590-1-AP

    Immunofluorescent analysis of Hela cells, using KRT6A antibody 10590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of A431 cells using Cytokeratin 6A Polyclonal antibody (10590-1-AP)

    FC experiment of A431 using 10590-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 6A (10590-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, HeLa cells, A375 cells, rat skin tissue, U-251 cells
Positive IHC detected inhuman oesophagus cancer tissue, human breast cancer tissue, human skin tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10590-1-AP targets Cytokeratin 6A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 6A fusion protein Ag0882
Full Name keratin 6A
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC008807
Gene Symbol Cytokeratin 6A
Gene ID (NCBI) 3853
RRIDAB_2134306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 6A antibody 10590-1-APDownload protocol
IHC protocol for Cytokeratin 6A antibody 10590-1-APDownload protocol
IF protocol for Cytokeratin 6A antibody 10590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Immunity

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Authors - Fangzhou Lou
View Article

Cell Rep

Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis

Authors - Jiaqing Hao
View Article
human,mouseIF

Cell Rep

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer

Authors - Xinyu Yang
View Article
mouseWB

Phytother Res

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Authors - Yongling Ning
View Article
human,mouseWB,IHC,IF

J Cell Mol Med

Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.

Authors - Lihua Gao
View Article
humanWB

J Eur Acad Dermatol Venereol

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.

Authors - T Yuan
View Article