Integrin beta-6-Specific Polyclonal antibody

Integrin beta-6-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19695-1-AP

Synonyms

Integrin beta 6, Integrin beta-6-Specific, integrin, beta 6, ITGB6



Tested Applications

Positive WB detected inA549 cells, A2780 cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19695-1-AP targets Integrin beta-6-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name integrin, beta 6
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberNM_000888
Gene Symbol Integrin beta 6
Gene ID (NCBI) 3694
RRIDAB_10644138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. The antibody is specific to ITGB6.

Protocols

Product Specific Protocols
WB protocol for Integrin beta-6-Specific antibody 19695-1-APDownload protocol
IHC protocol for Integrin beta-6-Specific antibody 19695-1-APDownload protocol
IF protocol for Integrin beta-6-Specific antibody 19695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat,mouseWB

Glia

αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo.

Authors - Kathleen K Catignas
humanIHC

Cell Biosci

Protein expression of eIF4E and integrin αvβ6 in colon cancer can predict clinical significance, reveal their correlation and imply possible mechanism of interaction.

Authors - Zhengchuan Niu
humanWB,IF,IP

J Exp Clin Cancer Res

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.

Authors - Feikai Lin
mouseIHC,IF

Mol Cell Endocrinol

Integrin β6 mediates epithelial-mesenchymal transition in diabetic kidney disease

Authors - Xiaoyu Li
humanWB,IHC

Adv Clin Exp Med

Upregulation of ITGB6 in primary palmar hyperhidrosis

Authors - Jian-Bo Lin
humanWB

Nat Commun

A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability

Authors - Ke Li