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Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IP | See 1 publications below |
| CoIP | See 3 publications below |
| ChIP | See 5 publications below |
Product Information
10284-1-AP targets Human IgG in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | Human IgG |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | 25 kDa, 50 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2877729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This rabbit poly clonal antibody recognizes the human IgG.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgG antibody 10284-1-AP | Download protocol |
| IHC protocol for Human IgG antibody 10284-1-AP | Download protocol |
| IF protocol for Human IgG antibody 10284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer. | ||
Int Immunopharmacol LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals. | ||
Front Bioeng Biotechnol Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes. | ||
Biosensors (Basel) Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber. | ||
Int J Oral Sci FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1. | ||
Clin Exp Metastasis Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Fatima (Verified Customer) (12-11-2018) |
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