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  • Featured Product
  • KD/KO Validated

KGA/GAM/GAC Polyclonal antibody

KGA/GAM/GAC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23549-1-AP

Synonyms

AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    WB analysis of HEK-293 using 23549-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    WB analysis of HeLa using 23549-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    WB analysis of HEK-293 using 23549-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    WB analysis of mouse brain using 23549-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    WB analysis of mouse liver using 23549-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    WB analysis of rat brain using 23549-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IP experiment of HEK-293 using 23549-1-AP

    IP result of anti-KGA/GAM/GAC (IP:23549-1-AP, 4ug; Detection:23549-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IHC staining of human kidney using 23549-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IHC staining of human kidney using 23549-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human brain tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IHC staining of human brain using 23549-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human brain tissue using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IHC staining of human brain using 23549-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IF Staining of HEK-293 using 23549-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KGA/GAM/GAC Polyclonal antibody (23549-1-AP)

    IF Staining of HeLa using 23549-1-AP

    Immunofluorescent analysis of HeLa cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23549-1-AP targets KGA/GAM/GAC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KGA/GAM/GAC fusion protein Ag20354
Full Name glutaminase
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight 58 kDa, 65 kDa, 83 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_2879295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.

Protocols

Product Specific Protocols
WB protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
IHC protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
IF protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
IP protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
  • KO Validated
View Article
humanWB

Redox Biol

Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.

Authors - M Ryan Smith
View Article
humanIHC,WB

Biosci Rep

Progesterone inhibits endometrial cancer growth by inhibiting glutamine metabolism through ASCT2

Authors - Jinqiu Guo
View Article
humanIF

Adv Sci (Weinh)

Overcoming Cancer Persister Cells by Stabilizing the ATF4 Promoter G-quadruplex

Authors - Chengmei Xiao
View Article