Tested Applications
| Positive WB detected in | pig colon tissue, human placenta tissue, human peripheral blood platelets, rat colon tissue, rabbit large intestine tissue, pig liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66325-1-Ig targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11105 Product name: Recombinant human F13A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 383-732 aa of BC027963 Sequence: RPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVIVEFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM Predict reactive species |
| Full Name | coagulation factor XIII, A1 polypeptide |
| Calculated Molecular Weight | 732 aa, 83 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC027963 |
| Gene Symbol | Factor XIIIa |
| Gene ID (NCBI) | 2162 |
| RRID | AB_2881706 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P00488 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Factor XIIIa antibody 66325-1-Ig | Download protocol |
| IHC protocol for Factor XIIIa antibody 66325-1-Ig | Download protocol |
| WB protocol for Factor XIIIa antibody 66325-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Factor XIIIa Monoclonal antibody (2D2B2), catalog number 66325-1-Ig, has a total of 3 citations. These appear in J Clin Invest, Phytomedicine, and Inflamm Regen. The research fields covered include autoimmune coagulation disorders and complement dysfunction, thrombosis and the intrinsic coagulation pathway, and inflammatory signaling in synovial tissue (rheumatology). Applications cited include ELISA, WB, and IHC in human and rat samples.
| Species | Application | Title |
|---|---|---|
J Clin Invest Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency. | ||
Phytomedicine Chinensinaphthol methyl ether prevents thrombosis by inhibiting the intrinsic pathway of coagulation through targeting coagulation factor XII. | ||
Inflamm Regen Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling |
