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EPHX2 Polyclonal antibody

EPHX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10833-1-AP

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of A549 using 10833-1-AP
WB result of EPHX2 antibody (10833-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.
WB analysis of HEK-293 using 10833-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 10833-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 10833-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat cerebellum using 10833-1-AP
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse large intestine using 10833-1-AP
IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:300) with mouse large intestine tissue lysate 3200 ug.
IP experiment of HEK-293 using 10833-1-AP
IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:500) with HEK-293 cells lysate 2800ug.
IHC staining of human colon cancer using 10833-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10833-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 10833-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10833-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10833-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10833-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 10833-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10833-1-AP (EPHX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10833-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, mouse colon tissue, rat cerebellum tissue, HEK-293T cells, A549 cells
Positive IP detected in	mouse large intestine tissue, HEK-293 cells
Positive IHC detected in	human colon cancer tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 14 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

10833-1-AP targets EPHX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EPHX2 fusion protein Ag1283
Full Name	epoxide hydrolase 2, cytoplasmic
Calculated Molecular Weight	63 kDa
Observed Molecular Weight	63 kDa
GenBank Accession Number	BC013874
Gene Symbol	EPHX2
Gene ID (NCBI)	2053
RRID	AB_2098246
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Protocols

Product Specific Protocols
WB protocol for EPHX2 antibody 10833-1-AP	Download protocol
IHC protocol for EPHX2 antibody 10833-1-AP	Download protocol
IF protocol for EPHX2 antibody 10833-1-AP	Download protocol
IP protocol for EPHX2 antibody 10833-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Gasdermin D-mediated metabolic crosstalk promotes tissue repair Authors - Zhexu Chi View Article
mouse	WB,IF	ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury Authors - Juan Zhang View Article
human	WB	Int J Biol Sci Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury Authors - Juan Zhang View Article
rat	WB	Biochim Biophys Acta Mol Basis Dis Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ Authors - Yang Zhou View Article
human	WB,IHC	Front Oncol EPHX2 Inhibits Colon Cancer Progression by Promoting Fatty Acid Degradation. Authors - Yiran Zhou View Article
chicken	WB	Arch Biochem Biophys Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase. Authors - Labitzke Erin M EM View Article

EPHX2 Polyclonal antibody

EPHX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A549 using 10833-1-AP

WB analysis of HEK-293 using 10833-1-AP

WB analysis of HEK-293T using 10833-1-AP

WB analysis of mouse colon using 10833-1-AP

WB analysis of rat cerebellum using 10833-1-AP

IP experiment of mouse large intestine using 10833-1-AP

IP experiment of HEK-293 using 10833-1-AP

IHC staining of human colon cancer using 10833-1-AP

IHC staining of human colon cancer using 10833-1-AP

IHC staining of rat kidney using 10833-1-AP

IHC staining of mouse brain using 10833-1-AP

IHC staining of mouse kidney using 10833-1-AP

IHC staining of mouse brain using 10833-1-AP

IF Staining of HEK-293 using 10833-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Gasdermin D-mediated metabolic crosstalk promotes tissue repair

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury

Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ

EPHX2 Inhibits Colon Cancer Progression by Promoting Fatty Acid Degradation.

Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase.