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  • Featured Product
  • KD/KO Validated

EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 18244-1-AP

Synonyms

EIF2AK2, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis using 18244-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis using 18244-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis of HepG2 using 18244-1-AP

    WB result of EIF2AK2, PKR antibody (18244-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK2, PKR transfected HepG2 cells.

  • Western Blot (WB) analysis of HeLa cells using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis of HeLa using 18244-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis of mouse thymus using 18244-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis using 18244-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    WB analysis of rat heart using 18244-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IP experiment of HeLa using 18244-1-AP

    IP result of anti-EIF2AK2, PKR (IP:18244-1-AP, 4ug; Detection:18244-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IHC staining of human kidney using 18244-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IHC staining of human kidney using 18244-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IHC staining of human kidney using 18244-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IHC staining of human kidney using 18244-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IHC staining of human testis using 18244-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IHC staining of human testis using 18244-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

    IF Staining of HeLa using 18244-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF2AK2, PKR antibody (18244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, rabbit, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK2, PKR fusion protein Ag13010
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated Molecular Weight 551 aa, 62 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC093676
Gene Symbol EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_2246451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IHC protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IF protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
IP protocol for EIF2AK2, PKR antibody 18244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
View Article
mouseWB

Nat Neurosci

Maternal immune activation in mice disrupts proteostasis in the fetal brain.

Authors - Brian T Kalish
View Article
humanWB

Sci Adv

Temporal control of the integrated stress response by a stochastic molecular switch.

Authors - Philipp Klein
View Article
humanWB

Nat Commun

NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.

Authors - Psylvia Léger
View Article
humanWB

J Immunother Cancer

Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment

Authors - Weiwei Lin
View Article
human,mouseWB

Int J Antimicrob Agents

Pan-viral propagation blockade by inhibiting host cell PNPT1

Authors - Shuang Qu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Heart