Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 18130-1-AP
IP result of anti-LC8/DYNLL1 (IP:18130-1-AP, 4ug; Detection:18130-1-AP 1:1000) with MCF-7 cells lysate 640 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18130-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18130-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 18130-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of Jurkat using 18130-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Positive FC (Intra) detected in
Jurkat cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
LC8 (also known as DYNLL1) is a homodimeric sequence-specific chaperone that promotes the ordered (typically homo-) oligomerization of more than a hundred protein targets. LC8/DYNLL1 has previously been proposed to function as an inhibitor of the NF-κB pathway, based on findings that it can bind directly to the IκBα regulatory region(PMID: 18579519). LC8/DYNLL1 regulates apoptotic activities of BCL2L11 by sequestering it to microtubules.
Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 18130-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18130-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18130-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18130-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of MCF-7 using 18130-1-AP
IP result of anti-LC8/DYNLL1 (IP:18130-1-AP, 4ug; Detection:18130-1-AP 1:1000) with MCF-7 cells lysate 640 ug.
IF/ICC Figures
IF Staining of MCF-7 using 18130-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of Jurkat using 18130-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
LC8/DYNLL1 Polyclonal antibody
Catalog Number
18130-1-AP
Citations
2
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
