CD68 Polyclonal antibody
CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
201
Cat no : 28058-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, mouse spleen tissue |
Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:600-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 38 publications below |
IHC | See 85 publications below |
IF | See 87 publications below |
FC | See 2 publications below |
Product Information
28058-1-AP targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Cited Reactivity | mouse, rat, pig, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD68 fusion protein Ag27827 |
Full Name | CD68 antigen |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | NM_001291058 |
Gene Symbol | Cd68 |
Gene ID (NCBI) | 12514 |
RRID | AB_2881049 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD68 antibody 28058-1-AP | Download protocol |
IHC protocol for CD68 antibody 28058-1-AP | Download protocol |
IF protocol for CD68 antibody 28058-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. | ||
J Pineal Res Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2. | ||
J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. | ||
J Exp Clin Cancer Res FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression | ||
Acta Biomater Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization | ||
Biomaterials Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alexandru (Verified Customer) (11-13-2023) | Great antibody, worked well in my experiments.
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FH Amelie (Verified Customer) (09-09-2019) | sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red
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