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  • Featured Product
  • KD/KO Validated

CTP synthase Polyclonal antibody

CTP synthase Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15914-1-AP

Synonyms

CTPS, UTP--ammonia ligase 1, EC:6.3.4.2, CTPS1, CTPS 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTP synthase Polyclonal antibody (15914-1-AP)

    WB analysis using 15914-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTP synthase Polyclonal antibody (15914-1-AP)

    WB analysis of HeLa using 15914-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CTP synthase Polyclonal antibody (15914-1-AP)

    IP experiment of HeLa using 15914-1-AP

    IP result of anti-CTP synthase (IP:15914-1-AP, 4ug; Detection:15914-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTP synthase Polyclonal antibody (15914-1-AP)

    IHC staining of human liver cancer using 15914-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTP synthase Polyclonal antibody (15914-1-AP)

    IHC staining of human liver cancer using 15914-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTP synthase Polyclonal antibody (15914-1-AP)

    IHC staining of human liver cancer using 15914-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CTP synthase Polyclonal antibody (15914-1-AP)

    IHC staining of human liver cancer using 15914-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTP synthase Polyclonal antibody (15914-1-AP)

    IF Staining of HepG2 using 15914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTP synthase antibody (15914-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CTP synthase Polyclonal antibody (15914-1-AP)

    FC experiment of HepG2 using 15914-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CTP synthase Polyclonal antibody (15914-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15914-1-AP targets CTP synthase in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish, mosquito
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTP synthase fusion protein Ag8707
Full Name CTP synthase
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC009408
Gene Symbol CTPS
Gene ID (NCBI) 1503
RRIDAB_2086513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

Protocols

Product Specific Protocols
WB protocol for CTP synthase antibody 15914-1-APDownload protocol
IHC protocol for CTP synthase antibody 15914-1-APDownload protocol
IF protocol for CTP synthase antibody 15914-1-APDownload protocol
IP protocol for CTP synthase antibody 15914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.

Authors - Zhe Sun
View Article
humanIF

FEBS J

Inter-filament interaction between IMPDH and CTPS cytoophidia.

Authors - Chia-Chun Chang
View Article
humanWB,CoIP

J Cell Physiol

Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization.

Authors - Ke Ma
View Article
human

Front Immunol

Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation.

Authors - Otavio Cabral-Marques
View Article
humanWB

Oncotarget

Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics.

Authors - Hong Qu
View Article
humanIF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise
View Article