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KD/KO Validated

CDK6 Polyclonal antibody

Name: CDK6 Polyclonal antibody
Brand: Proteintech
SKU: 19117-1-AP

CDK6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19117-1-AP

Synonyms

Serine/threonine-protein kinase PLSTIRE, EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 19117-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19117-1-AP
WB result of CDK6 antibody (19117-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.
IP experiment of Jurkat using 19117-1-AP
IP result of anti-CDK6 (IP:19117-1-AP, 4ug; Detection:19117-1-AP 1:1000) with Jurkat cells lysate 1960 ug.
IHC staining of human lung cancer using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 19117-1-AP (CDK6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 19117-1-AP (CDK6 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 19117-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (19117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 19117-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (19117-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 19117-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human lung cancer tissue, human gliomas tissue, human endometrial cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 15 publications below

Product Information

19117-1-AP targets CDK6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK6 fusion protein Ag5600
Full Name	cyclin-dependent kinase 6
Calculated Molecular Weight	326 aa, 36 kDa
Observed Molecular Weight	36-40 kDa
GenBank Accession Number	BC027989
Gene Symbol	CDK6
Gene ID (NCBI)	1021
RRID	AB_10640575
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 19117-1-AP	Download protocol
IHC protocol for CDK6 antibody 19117-1-AP	Download protocol
IF protocol for CDK6 antibody 19117-1-AP	Download protocol
IP protocol for CDK6 antibody 19117-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Theranostics Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes. Authors - Lei Wang View Article
human	WB	Cancers (Basel) A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches. Authors - Chiao-Yun Lin View Article
human	WB	Oncotarget High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma. Authors - Delong Meng View Article
human	WB	Front Pharmacol Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression. Authors - Yinshan Jin View Article
human	WB	Cancer Biother Radiopharm γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest. Authors - Zhifeng Lin View Article
human	WB	Cancer Med δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes. Authors - Lincheng Ju View Article

CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 19117-1-AP

WB analysis of HeLa using 19117-1-AP

IP experiment of Jurkat using 19117-1-AP

IHC staining of human lung cancer using 19117-1-AP

IHC staining of human lung cancer using 19117-1-AP

IHC staining of human endometrial cancer using 19117-1-AP

IHC staining of human endometrial cancer using 19117-1-AP

IHC staining of human gliomas using 19117-1-AP

IHC staining of human gliomas using 19117-1-AP

IF Staining of HeLa using 19117-1-AP

FC experiment of HeLa using 19117-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes.

A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.

γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest.

δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes.