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  • Featured Product
  • KD/KO Validated

CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 22060-1-AP

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK2 Polyclonal antibody (22060-1-AP)

    WB analysis using 22060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22060-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDK2 Polyclonal antibody (22060-1-AP)

    WB analysis of HeLa using 22060-1-AP

    WB result of CDK2 antibody (22060-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and CDK2 knockout HeLa cells.

  • Western Blot (WB) analysis of various lysates using CDK2 Polyclonal antibody (22060-1-AP)

    WB analysis using 22060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22060-1-AP (CDK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CDK2 Polyclonal antibody (22060-1-AP)

    IP experiment of HeLa using 22060-1-AP

    IP result of anti-CDK2 (IP:22060-1-AP, 4ug; Detection:22060-1-AP 1:1000) with HeLa cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDK2 Polyclonal antibody (22060-1-AP)

    IHC staining of human cervical cancer using 22060-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22060-1-AP (CDK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDK2 Polyclonal antibody (22060-1-AP)

    IHC staining of human cervical cancer using 22060-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22060-1-AP (CDK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK2 Polyclonal antibody (22060-1-AP)

    IF Staining of HeLa using 22060-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (22060-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CDK2 Polyclonal antibody (22060-1-AP)

    FC experiment of HeLa using 22060-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (22060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using CDK2 Polyclonal antibody (22060-1-AP)

    FC experiment of HepG2 using 22060-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (22060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22060-1-AP targets CDK2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK2 fusion protein Ag17133
Full Name cyclin-dependent kinase 2
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
RRIDAB_2878982
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919). It has 2 isoforms produced by alternative splicing with the molecular weight of 34 kDa and 30 kDa.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 22060-1-APDownload protocol
IHC protocol for CDK2 antibody 22060-1-APDownload protocol
IF protocol for CDK2 antibody 22060-1-APDownload protocol
IP protocol for CDK2 antibody 22060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Elife

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Authors - Andrew Kodani
View Article
human,mouseWB,IF

Biomark Res

Long noncoding RNA HOXC-AS3 interacts with CDK2 to promote proliferation in hepatocellular carcinoma

Authors - Chen Su
View Article
humanIF

Eur J Pharmacol

Hypericin-mediated photodynamic therapy inhibits growth of colorectal cancer cells via inducing S phase cell cycle arrest and apoptosis.

Authors - Jinhang Hu
View Article
ratWB

Front Pharmacol

Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres

Authors - Shaomin Wang
View Article
humanWB

Am J Cancer Res

E2F1-mediated GINS2 transcriptional activation promotes tumor progression through PI3K/AKT/mTOR pathway in hepatocellular carcinoma

Authors - Yao Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,