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CD41/Integrin alpha 2b Monoclonal antibody

CD41/Integrin alpha 2b Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6D7C5

Cat no : 60350-1-Ig

Synonyms

CD41, CD41/Integrin alpha 2b, CD41B, GP2B, GPalpha IIb, GPIIb, GTA, HPA3, Integrin alpha IIb, ITGA2B, ITGAB

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using CD41/Integrin Alpha 2B Monoclonal antibody (60350-1-Ig)

    WB analysis of human spleen using 60350-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60350-1-Ig (CD41/Integrin alpha 2b Antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60350-1-Ig targets CD41/Integrin alpha 2b in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC126442
Gene Symbol CD41a
Gene ID (NCBI) 3674
RRIDAB_2881459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.

Protocols

Product Specific Protocols
WB protocol for CD41/Integrin alpha 2b antibody 60350-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

In-situ gelation of fibrin gel encapsulating platelet-rich plasma-derived exosomes promotes rotator cuff healing

Authors - Molin Li
View Article