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Cyclin B1 Polyclonal antibody

Name: Cyclin B1 Polyclonal antibody
Brand: Proteintech
SKU: 55004-1-AP

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

262

Review Product

Cat no : 55004-1-AP

Synonyms

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55004-1-AP
Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
WB analysis of HeLa using 55004-1-AP
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
WB analysis of HeLa using 55004-1-AP
WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.
WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 55004-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 55004-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55004-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 55004-1-AP
IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug.
IHC staining of human skin cancer using 55004-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 55004-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 55004-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of U2OS using 55004-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HeLa using 55004-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 55004-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, U2OS cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 248 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

55004-1-AP targets Cyclin B1 in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	cyclin B1
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	55-60 kDa
GenBank Accession Number	NM_031966
Gene Symbol	Cyclin B1
Gene ID (NCBI)	891
RRID	AB_10859790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IHC protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IF protocol for Cyclin B1 antibody 55004-1-AP	Download protocol
IP protocol for Cyclin B1 antibody 55004-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration Authors - Jinpeng Du View Article
human	WB	Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis Authors - Wei Zhang View Article
human	WB	Acta Pharm Sin B Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway. Authors - Xiangjin Zheng View Article
mouse	IF	Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. Authors - Weimin Yu View Article
human	WB	Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Authors - Xiaoyu Ma View Article
human	WB	Autophagy PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma. Authors - Bo Wang View Article

Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

WB analysis using 55004-1-AP

WB analysis of NIH/3T3 using 55004-1-AP

WB analysis using 55004-1-AP

WB analysis of HeLa using 55004-1-AP

IP experiment of HeLa using 55004-1-AP

IHC staining of human skin cancer using 55004-1-AP

IHC staining of human skin cancer using 55004-1-AP

IF Staining of HeLa using 55004-1-AP

IF Staining of U2OS using 55004-1-AP

FC experiment of HeLa using 55004-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.

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