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  • Featured Product
  • KD/KO Validated

Caspase 8/p43/p18 Polyclonal antibody

Caspase 8/p43/p18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 13423-1-AP

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis of Jurkat using 13423-1-AP

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis using 13423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis of RAW264.7 using 13423-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of UV treated Hela using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis of UV treated Hela using 13423-1-AP

    UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis of Raji using 13423-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis of RAW264.7 using 13423-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    WB analysis of HeLa using 13423-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IP experiment of HeLa using 13423-1-AP

    IP result of anti-Caspase 8/p43/p18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

  • Immunoprecipitation (IP) experiment of Jurkat cells using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IP experiment of Jurkat using 13423-1-AP

    IP result of anti-Caspase 8/p43/p18 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human lymphoma using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human colon using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IHC staining of human colon using 13423-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Caspase 8/p43/p18 Polyclonal antibody (13423-1-AP)

    IF Staining of HepG2 using 13423-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/p43/p18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inStaurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13423-1-AP targets Caspase 8/p43/p18 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, rabbit, chicken, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag4214
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated Molecular Weight 538 aa, 62 kDa
Observed Molecular Weight 53-57 kDa, 32-45 kDa
GenBank Accession NumberBC028223
Gene Symbol Caspase 8
Gene ID (NCBI) 841
RRIDAB_2068463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
IHC protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
IF protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
IP protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
View Article
IP

Sci Adv

HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.

Authors - Chun Gao
View Article
humanWB

Exp Mol Med

PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer

Authors - Gu-Choul Shin
View Article
humanWB

Nat Commun

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.

Authors - Christoph Grohmann
View Article
humanWB

Aging Dis

MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.

Authors - Yanhui Cui
View Article
humanWB

EMBO J

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Xiaoping (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:500 dilution

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: kidney
FH

WEI (Verified Customer) (01-17-2020)

Good antibody for detecting IR injury-induced apoptosis in mouse heart.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart