Tricine Gel Recipe For Low Molecular Weight Proteins

Tricine SDS-PAGE gel recipe for western blot, for target proteins less than 20 kDa.

For target proteins with MWs of less than 20 kDa, a tricine gel system will obtain higher resolution and is highly recommended. Make three layers of tricine gels as laid out in the following table and diagram. Apply specific tricine gel running buffer to the running system and perform transfer as usual.

Recipe 1

Items	Stacking	Intermediate	Separation
Gel Percentage	4%	10%	15%
Gel Volume	2ml	3ml	6ml
38% Glycerol	-	-	1.6
ddH20	1.4	1.2	-
30% Acrylamide	0.3	0.8	2.7
3.0 M Tris HCl (pH 8.5)	-	1	2.14
1.0 M Tris HCl (pH 6.8)	0.3	-	-
10% SDS	0.02	0.03	0.06
10% APS	0.02	0.03	0.06
TEMED	0.002	0.003	0.003