Standard Workflow
Labeling 0.5µg of primary antibody
Before you begin, equilibrate all reagents to room temperature
Procedure:
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Combine 0.5µg of primary antibody with 1µL of FlexLinker. Add FlexBuffer to bring total volume to 8µL.
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Mix gently and incubate for 5 minutes at room temperature in the dark.
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Add 2µL of FlexQuencher.
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Mix gently and incubate for 5 minutes at room temperature in the dark.
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The antibody is now ready to be used.
Scale up proportionately to label larger quantities of primary antibody
You can scale up the required volumes proportionally according to the amount of primary antibody required.
Antibody quantity |
0.5 µg |
1 µg |
2.5 µg |
5 µg |
10 µg |
25 µg |
FlexLinker Volume |
1 µL |
2 µL |
5 µL |
10 µL |
20 µL |
50 µL |
FlexBuffer Volume |
≤ 8 µL |
≤ 16 µL |
≤ 40 µL |
≤ 80 µL |
≤ 160 µL |
≤ 400 µL |
FlexQuencher Volume |
2 µL |
4 µL |
10 µL |
20 µL |
40 µL |
100 µL |