Non-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.
TDP-43 (C-terminal) Polyclonal antibodies
(Parent antibody plus 3 derived formats/conjugates)
KO/KD Validated
247 Publications
Reviews (9)
Host/IsoType
Rabbit/IgG
Reactivity
human, mouse, rat, chicken, drosophila, monkey, zebrafish
Applications
ChIP, CoIP, FC (Intra), IF/ICC, IF-Fro, IHC, IP, RIP, WB
Conjugation
Biotin, CoraLite® Plus 488, CoraLite®594, Unconjugated
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
K562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-TDP-43 (IP:18280-1-AP, 3ug; Detection:18280-1-AP 1:1500) with K-562 cells lysate 6000ug.
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (18280-1-AP) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug TDP-43 (full length) Polyclonal antibody (18280-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).