Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells.

A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP (TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-P53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug.

Immunofluorescent analysis of HepG2 cells using 21891-1-AP (TP53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Non-treated HT-29, phosphatase inhibitor treated and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28960-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28959-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

Non-treated HEK-293, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 28963-1-AP (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Standard curve of Human P53 ELISA Kit KE00244.

Standard curve of Human Phospho-P53 (Ser15) ELISA Kit KE40001.

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using KHC0079 (P53 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC0079 (P53 IHC Kit).

Structure of VHH.

P53 fusion protein Ag0698 SDS-PAGE

Structure of VHH.