Purity of recombinant human IL-29 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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Recombinant human IL-29 (HZ-1156) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range is 0.12-0.6 ng/mL.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Lambda Light Chain antibody (20758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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human plasma were subjected to SDS PAGE followed by western blot with 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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80 nL human plasma were subjected to SDS PAGE followed by western blot with 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG lambda chain antibody (67762-1-Ig, Clone: 1D12B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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human plasma were subjected to SDS PAGE followed by western blot with HRP-20758 (IgG Lambda Light Chain antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-67762 (Human IgG lambda chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-11541 (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-20758 (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®488 Human IgG lambda chain antibody (CL488-67762, Clone: 1D12B5 ) at dilution of 1:200.

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Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®488 IgG Lambda Light Chain antibody (CL488-11541) at dilution of 1:200.

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