Purity of recombinant human IL-29 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-29 (HZ-1156) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range is 0.12-0.6 ng/mL.

Purity of recombinant human IL-28B was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-28B (HZ-1245) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 0.07-0.35 ng/mL.

Purity of recombinant human IL-28A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-28A (HZ-1235) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.01-0.06 ng/mL.

Purity of recombinant human IL-28A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-28A (HZ-1235) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.01-0.06 ng/mL.

BLI analysis of the capture of varying concentrations of HumanKine IL-28A (HZ-1235) by biotinylated anti-IL-28A VHH (IL28a-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

ELISA analysis of the capture of varying concentrations of HumanKine IL-28A (HZ-1235) by biotinylated anti-IL-28A VHH (IL28a-b). Detection via rabbit polyclonal PTG 22646-1-AP.

BLI analysis of the interaction between varying concentrations of anti-IL-28A VHH and immobilised, biotinylated HumanKine IL-28A (HZ-1235). Fit indicates fitting of data to a 1:1 binding model.

Standard curve of AuthentiKine™ Human IL-28A ELISA Kit KE00237.

Standard curve of AuthentiKine™ Human IL-28A ELISA Kit KE00237.

Standard curve of AuthentiKine™ Human IL-29 ELISA Kit KE00241.

Standard curve of AuthentiKine™ Human IL-29 ELISA Kit KE00241.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0073 (Human IgG lambda chain IHC Kit).

Recombinant protein were subjected to SDS PAGE followed by western blot with 22646-1-AP (IL-28A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).