Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-Tubulin alpha antibody, labeled with FlexAble Biotin Kit (KFA128) and Streptavidin-647 (magenta). Cell nuclei were stained with DAPI (blue).  This epifluorescence image was acquired at Zeiss Demo Center, Munich, with a 40x objective and post-processed. 

Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG2b kappa anti-RNA PolymeraseII antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 488 for Rat Kappa Light Chain (KFA121) and CD3 antibody (CL750-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 488 for Rat Kappa Light Chain (KFA121). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 488 Kit (KFA121) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).   Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2b kappa anti-RNA Polymerase II antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red). Cell nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 555 for Rat Kappa Light Chain (KFA122) and CD3 antibody (CL750-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 555 for Rat Kappa Light Chain (KFA122). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 555 Kit (KFA122) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG2b kappa anti-RNA PolymeraseII antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2b kappa anti-RNA Polymerase II antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).   Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescent analysis of FFPE mouse colon tissue slides with Rat anti-mouse Cdh1 antibody labeled with FlexAble CoraLite Plus 647 Kit (KFA123, magenta). With/without quencher. Cell nuclei were stained with DAPI (blue).

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). The cells were surface stained with CD3 antibody (CL488-65151) before fixation. Then, cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 647 kit (KFA123).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1) or rat IgG2a isotype control (65209-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed. Histogram, red: Rat IgG2a isotype control (65209-1-Ig); blue: Rat anti-mouse CD86 (65068-1-Ig).

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite Plus 488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 647 (KFA123). Cells were not fixed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 647 for Rat Kappa Light Chain (KFA123) and CD3 antibody (CL488-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 647 for Rat Kappa Light Chain (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 488 Kit (KFA121) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble FITC Plus Kit (KFA126) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 555 Kit (KFA122) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with rat anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.