1x10^6 C57BL/c mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Foxp3 (3G3) (65089-1-Ig, Clone: 3G3) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), FITC anti-Mouse IgG1 Antibody, and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1x10^6 PMA and ionomycin treated Th17-polarized splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IL-17A (230038C7) Rabbit Recombinant Antibody (CL488-98005, Clone:230038C7), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5), and 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug PE Anti-Mouse IFN gamma (PE-65153, Clone: XMG1.2).
1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65187-1-Ig, Clone:Y1/82A) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD68 antibody (65187-1-Ig, Clone: Y1/82A ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107a / LAMP1 (65050-1-Ig, Clone:1D4B) and FITC anti-Rat IgG2a antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65050-1-Ig (CD107a antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).
1X10^6 BALB/c mouse splenocytes were surface stained with PE Anti-Mouse CD4 (GK1.5) and then fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. Cells were then intracellularly stained with 0.06 ug APC Anti-Mouse Foxp3 (APC-65089, Clone:3G3) or 0.06 ug APC Mouse IgG1 Isotype Control (MOPC-21). It is recommended that the antibody dosage should not exceed 0.06ug.
1X10^6 unstimulated and anti-CD3/CD28-stimulated (with 50 ng/ml TGF-β, 3 days) mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), and then intracellularly stained with 0.06 ug PE Anti-Mouse Foxp3 (PE-65089, Clone:3G3).
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD107a / LAMP1 (CL647-65050, Clone:1D4B) (red), or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 647 CD107a antibody (CL647-65050, Clone: 1D4B ) at dilution of 1:400.
1X10^6 NIH/3T3 cells were intracellularly stained with 4 ul FITC Anti-Mouse CD107a / LAMP1 (FITC-65050, Clone:1D4B) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b / LAMP2 (65052-1-Ig, Clone: ABL-93) and FITC anti-Rat IgG2a Antibody (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65052-1-Ig (CD107b antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using APC-65050 (CD107a antibody) at dilution of 1:100.
1X10^6 NIH/3T3 cells were intracellularly stained with 10 ul APC Anti-Mouse CD107a (APC-65050, Clone:1D4B) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD107a / LAMP1 (CL488-65050, Clone:1D4B) or 0.5 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 CD107a antibody (CL488-65050, Clone: 1D4B ) at dilution of 1:400.
1x10^6 mouse splenocytes were intracellularly stained with 0.06 ug CoraLite® Plus 488 Anti-Mouse Foxp3 (CL488-65089, Clone:3G3), and 0.06 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5), and 0.06 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107a (CL647-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107a antibody (CL647-65051, Clone: H4A3) at dilution of 1:400. F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).
1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.
1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.
1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.
1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of Brefeldin A and Monensin) Th1-polarized splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug FITC Plus Anti-Mouse IFN gamma (FITC-65153, Clone: XMG1.2).
1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.
1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.
1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th2-polarized splenocytes were surface stained 0.2 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.125 ug PE Anti-Mouse IL-4 (PE-65074, Clone: 11B11).
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-MR, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).