Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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Recombinant human IL-5 (HZ-1324) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-5 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-0.9 ng/mL.

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Recombinant protein were subjected to SDS PAGE followed by western blot with 26677-1-AP (IL5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Standard curve of Human IL-5 ELISA Kit KE00304.

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Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 30198-1-AP (IL-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IL-5 fusion protein Ag24664 SDS-PAGE

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human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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