Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22226-1-AP (NOS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

1X10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (22226-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

mouse testis tissue were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 40x lens).

Immunofluorescent analysis of HepG2 cells, using ISYNA1 antibody 14142-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).