1X10^6 mouse splenocytes were surface stained with CoraLite®488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with 0.13 ug APC-mouse IgG1 isotype control or 0.13 ug APC Anti-Mouse Foxp3 (APC-65089, Clone: 3G3).