1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 1 ug FITC Plus Anti-Mouse CD3 (FITC-65077, Clone: 17A2) or 1 ug isotype control. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug FITC-Anti-Mouse CD3ε (FITC-65060, clone 145-2C11) (red) or 0.20 ug FITC-Hamster IgG isotype control (blue). Cells were not fixed.

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1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD8a (65144-1-Ig, Clone:RPA-T8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD8a (APC-65069, Clone:53-6.7) or 0.2 ug Isotype Control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug PE Anti-Mouse CD8a (PE-65069, Clone:53-6.7) or 0.5 ug Control Antibody. Cells were not fixed.

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1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (APC-65104, Clone: GK1.5), and 0.5 ug Anti-Mouse CD8a (65069-1-Ig, Clone: 53-6.7) and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (APC-65104, Clone: GK1.5), and 0.5 ug Rat IgG2a Isotype Control and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite®594 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) or 0.5 ug Isotype Control. Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) at dilution of 1:500.

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