1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Human CD81 (CL647-65195, Clone: 5A6) (blue) or isotype control antibody (black). Cells were not fixed.
1X10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70) or unstained. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 1 ug FITC Plus Anti-Mouse CD3 (FITC-65077, Clone: 17A2) or 1 ug isotype control. Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug FITC-Anti-Mouse CD3ε (FITC-65060, clone 145-2C11) (red) or 0.20 ug FITC-Hamster IgG isotype control (blue). Cells were not fixed.
1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Anti-Mouse CD80 (B7-1) (FITC-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1). Cells were not fixed.
1X10^6 LPS treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1) (red) or 0.125 ug FITC-rat IgG2a isotype control. Cells were not fixed.
